The present study was carried out to determine the extent of haemato-biochemical alterations in 28 days repeated dose oral toxicity of sodium fl uoride (NaF) at three dose levels in Wistar rats. The administration of NaF caused dose dependent reduction (p ≤ 0.01) in the levels of haemoglobin, packed cell volume, total erythrocyte count and lymphocyte percent while an increase in mean corpuscular volume, mean corpuscular haemoglobin and neutrophils of all the rats belonging to group II (5 mg/kg body weight, per os), group III (25 mg/kg body weight, per os) and group IV (50 mg/kg body weight, per os) as compared to rats of control group (group I). Macrocytic- normochromic anaemia was recorded in NaF intoxicated rats. Biochemical alterations were dose dependent elevation (p ≤ 0.01) in plasma enzyme activities of alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase in group II, III and IV in comparison to rats of control group. Hyperglycaemia (p < 0.01) was recorded in rats of group III and IV. Hypoproteinaemia (p < 0.01) and hypoglobulinaemia (p < 0.01) were seen in Wistar rats of group II, III and IV as compared to control group. Moreover, increased (p < 0.05) albumin to globulin ratio, blood urea nitogen, creatinine and uric acid levels were observed in the intoxicated rats of group II, III and IV as compared to control group. Thus, it is concluded that 28 days subacute toxicity of NaF in wistar rats has adverse effects on haemato-biochemical profi le.